Training in infection prevention and control (IPC) measures is crucial to minimise the incidence of healthcare-associated infections (HAIs), a growing cause of patient illness and death in hospital. This paper describes a participative approach to developing a prototype tablet-based digital training tool using dynamic visualisation-led techniques to raise awareness and understanding of IPC and HAIs for hospital-based staff. An evidence-based and iterative visualisation prototyping process was used to engage staff and invite contributions from across a number of roles within the NHS, a typically hierarchical sector. Findings suggest the visualisation-led approach was helpful in articulating the behaviours of pathogens and staff and their interactions within the complex setting and service ecology of the NHS and in making IPC training materials clearer and more engaging.